APOPTOSIS AND DNA CHANGES IN HEPATOCYTES AFTER SELECTIVE PORTAL VEIN EMBOLIZATION; CONTROLLED EXPERIMENTAL STUDY

Document Type : Original Article

Authors

1 Departments of General Surgery & Experimental Unit

2 Pathology, Theodore Bilharz Research Institute

Abstract

Introduction: Extensive hepatic resection could be done by performing pre-operative portal vein embolization (PVE), to 
induce compensatory hypertrophy in the predicted remnant liver. The aim of this study is to evaluate the effect of selective (PVE) on hepatocytes. 
Methods: The study was done on 23 adult Shin Sheila rabbits, embolization of the lateral right branch of the portal vein 
using Histoacryl was done. The animals were divided into 2 groups: (group-A) ; 12 animals sacrificed in < 30 days and (groupB); 11animals sacrificed between 30 to 60 days. Biopsies were taken from embolized (E) and non embolized (NE) lobes and examined . 
Results: Degeneration was remarkable in (E) lobe in (group-A), while it was not noticeable in (group-B). Regeneration was evident in (E) lobe in (group-B). Proliferation occurred in (NE) lobes in (group-A) in the (NE) lobes. Regeneration was marked in (group-B) in the (E) lobe. Morphometric DNA study showed apoptosis in the (E) lobe of (group-A) were more than in the (NE) lobes while in (group-B) they were nearly the same in both lobes. 
Conclusion: PVE induce apoptotic changes in (E) lobe in the early stage this was re-compensated by increased cellular 
proliferation in late stage evident by increased in S phase fraction of DNA by image analysis study.

Keywords

The Egyptian Journal of Surgery
Volume 23, Issue 4
October 2004
Pages 366-372

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