Lack of association between factor-associated suicide gene-670 A/G polymorphism and risk of breast cancer in Egyptian women

Background
Breast cancer (BC) is among the commonest malignancy and is the leading cause of women’s death globally. Factor-associated suicide (FAS) is a key player in the initiation of apoptosis. A to G single nucleotide polymorphism (SNP) at 670 bp in the FAS gene promoter diminishes the interaction of transcription factors with the promoter and the level of FAS expression.
Patients and methods
This study investigated whether the FAS gene promoter A/G SNP at 670 bp increases the risk of BC in Egyptian females. A total of 300 patients with BC and 300 healthy controls were enrolled in this case-control research. To assess genotyping, DNA taken from participants’ blood was subjected to PCR-restriction fragment length polymorphism procedure.
Results
FAS-670 A/G genotypes and alleles had no significant association with BC risk ( value for genotypes and alleles was 0.250 and 0.164, respectively), but women with the GG genotype who use contraception have a 4.74 times higher risk of developing BC than persons with the AA genotype who do not use contraception. Furthermore, contraceptive users with the GG genotype have a 3.5 times higher chance of developing BC than noncontraceptive users with the same genotype. In addition, the distribution of genotypes and alleles in different BC stages was significant statistically (=0.001).
Conclusion
There is a lack of association between the FAS-670 A/G SNP and BC risk in Egyptian women. According to this study, women with the GG genotype who used contraception had a statistically significant chance of developing BC. Furthermore, the FAS-670 A/G SNP was associated with BC progression in a statistically significant manner.